The purpose of this lab is to extract trimyristin from nutmeg with the organic substance, diethyl ether, to evidently produce trimyristin with a small portion of myristicin solid-liquid extraction. Hydrolysis and acidification make glycerol and myristic acid. For this experiment standard solutions of Kool-Aid flavors will be prepared to measure the absorbance at lambda max and plot the results against the concentration of solution to generate a standard curve. When we were to extract myristic acid from. Although nutmeg is one of those natural sources that trimyristin is easily extracted from it does take some time to achieve in lab. Then we used the fritted funnel with Celite, which is a filter aid inside the filter for filtration.
I secured the flask to the three-pronged clamp on the stand over the sand bath to heat for 45 minutes. Physical Data List the molecular weight, melting point, boiling point, density, solubility, and hazards of all pertinent chemicals used in the experiment. This eventually leads to him making up voices of scorn and pity. We can state however, that the impurity was not by a lot since we were fairly close to the literature value. The organic solvents that were used in the experiment were diethyl ether, methylene chloride and ethyl acetate. Also, it is good to know that due to impurity the best way purify the product of trimyristin is by recrystallization through hot acetone.
Vacuum filter uses the thick vacuum tubes, not thin ones! The remains were the yellow solid of crude trymyristin mentioned in the lab description. Plant seeds are typically rich in triglycerides, the fatty acid triesters of glycerol. The remainder should be left to air dry for 5 minutes and the weight was recorded to be 24. This experiment involves extraction and separation techniques that yield a purified product. Trimyristin does not dissolve in water, a characteristics it shares with various other fatty acids and oily compounds However, it dissolves quite easily in chloroform, ethanol, benzene, ether and dicloromethane. After the myristic acid is collected we will be using its melting point to determine whether we successfully isolated pure form of the crystal. I got almost no crystals in the second recrystallization; they were visible, but not plenty enough to be put into the mel-temp device.
Separation using organic solvent is mostly utilized in pharmaceutical industry. The mixture was allowed to settle, and the liquid was filtered in the same pressure filtration apparatus. The experimental yield was 0. After the hydrolysis, I let the solution cool, poured 8mL of distilled water into a 50mL Erlenmeyer and poured the solution into another flask. We will be looking specifically at an organic solvent extraction which is a type of solid-liquid extraction. This is a result of a loss of the samples during the partition process, reflux, as well as recrystallization. A small amount of the sample was saved for the Melting Point, which was found to be 50-52 degrees Celsius.
The apparatus is set up by pouring vegetable oil from the bottle into the Thiele tube. The technique used was steam distillation. If the mixture were heated at 40° for 45 minutes, what would be the result 1. Some changes were also made to step 12. For future experiments I should work to reduce random errors in my measurements and improve my technique to reduce impurities and improve yields.
Caffeine is a member of the class of compounds organic chemists call alkaloids. It was also proven that trimyristin is soluble in acetone, the substance we used for recrystallization. I clamped this to the ring stand and let it boil for 10 minutes. From that point, a capillary tube is loaded with the fine powder by tapping the open end of the tube into the glass plate which the powder is located. This is very convenient for the chemist whom want the reaction to be thermically accelerated at a higher temperature without worrying about adding excess reagents unless needed to. Two milligrams of it is measured and placed into a watch glass by which grams scale.
This type of extraction utilizes an organic solvent that has a high affinity for the compound of interest. Table of Reagent: Structure Mw. Take the necessary and be patient and calm in order to avoid unfortunate accidents with chemicals and fire. A minimal amount of methylene chloride was then used to wash any excess residue out of the round bottom flask and into the filter. This could have been from not waiting long enough for crystals to form, or a small amount of Trimyristin stayed dissolved in the solution. In the experiment, we performed with microscale instead of a standard scale.
Or, you can find the. Mihordea Motion of Atoms and Molecules lab summary In this lab the standard heat of combustion of magnesium was calculated by using the calorimeter. The change in culture sparked a change in cinema that appealed to a younger audience. Table of Pertinent Physical Constants % of Trimyristin in Dried Weight of Ground Nutmeg 20-25% Literature Value Melting Point of Trimyristin 56-57°C Boiling Point of Methylene Chloride 39. You should assume that all compounds encountered in the laboratory are toxic and handle them accordingly.
After the myristic acid is collected we will be using its melting point to determine whether we successfully isolated pure form of the crystal. It is then recrystallized from acetone to pure Trimyristin. This is important to avoid forgetting important details. Also, the point of combustion of magnesium was to see how much heat would be released from fireworks such as sparklers. Myristicin poisoning can induce convulsions, palpitations, nausea, eventual dehydration, and generalized body pain.