Materials: Baking soda Liquid soap Plastic syringes 10 mL or larger Leaves spinach or ivy Hole punch or Straws Cups or beakers Timer Light source Procedure 1. Leaf structures will be named and discussion will relate these structures to the processes of photosynthesis that occur in each. After two days, we measured the amount of light going through the substance to see how much photosynthesis had occurred. The first step was to cut a point on one end of the filter paper and draw a pencil line 1. The teacher can now review what a hypothesis is and how to write a specific, testable hypothesis. On the order hand, an electron microscope uses electrons and electromagnetic condensers. Chlorophyll b makes up about 25% of the pigmentation.
The energy from visible light is used in the photosynthetic process. Putting in too much sodium bicarbonate. A redox reaction is oxidation and reduction. Learning Goals This activity is designed for students to: 1 learn that the rate of photosynthesis is influenced by environmental factors that can be quantified 2 understand the equation of photosynthesis and how the structure of a leaf allows for the required gas exchange to occur through the stomata 3 properly design an experiment with one variable, analyze results and report findings to the class Students will use higher order thinking skills throughout this activity. The spongy mesophyll layer is normally infused with gases, oxygen and carbon dioxide. Materials: test tube, Elodea cuttings, sodium bicarbonate baking soda , beaker with water, lamp Part 1: Measurement of Photosynthesis There are various set-ups that can be used to measure the rate of photosynthesis, each relies on counting the oxygen produced during the reaction. In order to make sugar plants require only the following: light energy, water, and carbon dioxide.
Experimental Question: What factors affect the rate of photosynthesis? This gives a green line across the paper, which contains the pigments of the leave. Once they have sank, you can put them back into the sodium bicarbonate solution and expose the disks to light. Photosynthesis occurs in the chloroplast, an organelle in plant cells that contains the molecule chlorophyll. The leaves in the bicarbonate solution did eventually float up, following the. How does carbon dioxide get into a leaf? Like all enzyme-driven reactions, the rate of photosynthesis can be measured by either the disappearance of substrate, or the accumulation of products. This lab experiment is about studying the inheritance of Zea Mays kernel color and texture similar to the one Gregor Mendel performed in his experiments on heredity in corn seed color and seed shape.
Replace the plunger and push out most of the air, but do not crush your leaves. What types of organisms undergo photosynthesis? When in the presence of sodium bicarbonate leaf discs immediately started to float up. Lab Report Include the following sections in your lab report, which should be typed. Materials and Methods: Lab 4A: The materials used in this section of the lab were: filter paper, glass vial, small amount of solvent, a quarter, and spinach leaves. From the first to the second trial with water added onto leaves, oxygen increased by 63. Objective : To observe and investigate the cell structure under the light microscope. A light microscope utilizes light that can be altered by a filter and condenser to visualize a specimen.
Pick a testable question to answer when doing the experiment. Then, as photosynthesis and respiration occur you can find the net rate of photosynthesis occurring in a leaf over some time. You will measure how long it takes in seconds for the disks to float as a way to measure the rate of photosynthesis. Abstract For this lab the rate of photosynthesis was analyzed upon plant leaves. Question: How does water vs.
So what does the data say? Your experiment created a reaction called an Exothermic Reaction - that means it not only created foam, it created heat! Some possible mistakes that could have occurred during this experiment can include the following: Miscalculating the distance between the Elodea plant and the light source. Leaf Structure and Function In this investigation, you will use a system that measures the accumulation of oxygen in the leaf. The dependent variable was the speed at which the leaf disks started to float. This activity will allow students to measure the rate at which the photosynthesis process occurs. This process has two subparts… 1. One improvement to the experiment would be keeping precise measurements of the amount of distilled water added or the amount of sodium bicarbonate mixed in order to keep any accurate results.
There are three types: Column, Paper, and Thin Layer chromatography. Suck up about 10 cc's of water. The foam produced is just water, soap, and oxygen so you can clean it up with a sponge and pour any extra liquid left in the bottle down the drain. There will be no starting point and thus it is hard to validate any change in data. Leaf disks were monitored for 15 minutes to see if they would float to the top of the solution. This process takes place in the leaves of a plant specifically in the chloroplast and it uses chlorophyll. Discuss the experimental set-up and how it can be used to measure the rate of photosynthesis.
We will determine whether or not it is possible to examine the relationship between photosynthesis and cellular respiration under controlled environmental conditions. In order to fulfill the object of the experiment, we blended the spinach with 3 solutions. Background and PreLab Photosynthesis fuels ecosystems and replenishes the Earth's atmosphere with oxygen. This could mean that substrate below. Also, the plant was heated up towards the end and it gave results, even though the distance between the plant and lamp was greater. Hypothesis After reading the lab instructions but before starting the lab record your best educated guess about what will happen in the experiment. Introduction Every species on earth needs some kind of energy sources in order to survive.
The dependent variable was the speed at which the leaf disks started to float. Both… Color and Photosynthesis Rate Introduction: How do the wavelength of visible light affect photosynthesis? There is stroma and grana and thylakoid. Get the first maize 2. The solvent front was then quickly marked with a pencil and then each pigment front was marked as well. Your goal is to find a way to consistently measure the rate of photosynthesis using any of the designs above. Introduction: Explain photosynthesis and include the chemical equation.
Which of the 2 types of chlorophyll is more soluble? It increases magnification, resolution, and contrast. Then at minute 20 all of the discs were floating. In today's lab, we shall focus on the principles, parts and functions of a bright-field light microscope. With the members of your group, brainstorm variables which may affect the rates of photosynthesis. The Oxygen deprived leaf disks were placed under a light source to mimic the suns effect. The weight of each of the insects was also a factor in determining the rate of respiration.