Bial's - tests for furanose ring. Each organic compound has a specific role and function in our body. Introduction The labs conducted tested the samples of saltine cracker and lemonade power for all organic compounds including, lipids, carbohydrates, proteins and simple sugars. I removed the test tubes from the bath and recorded the color changed in table 1. The alcoholic group can be detected by the following tests: 1.
Fill test tube B with 3 pipettes full of substance B. Lipids are basically the… 2953 Words 12 Pages Organic Lab 1: Fractional Distillation Discussion: With the purpose of the experiment being to identify the 30 mL of unknown liquid, the theoretical basis of simple and fractional distillation must be deconstructed and applied to the data obtained describing the liquid in question. Carboxylic acids such as benzoic acid, oxalic acid, phthalic acid, tartaric acid etc are colourless crystalline solids. Carbohydrates monomers are called monosaccharides ex; fructose, and galactose. Sucrose is a disaccharide that consists of glucose and galactose. Carbohydrates give energy to cells when consumed.
If the test samples show the presence of lipids, the when. Place the tubes in a boiling water bath for 3 minutes. Tests for Carboxylic group Carboxylic acids are organic compounds containing carboxyl functional group. Fill test tube 1 up to the 1cm mark with distilled. This test is used to differentiate between ketoses and aldoses. This lab gave us the opportunity to observe the compounds evident in the egg yolk and the egg white. Starch reacted to iodine along with the potato proving starch present.
It is of two types aliphatic and aromatic. Benedicts reagent contains sodium bicarbonate, sodium citrate and copper sulfate. Glycogen, the common polysaccharide in animals, has a slight difference in structure and produces only an intermediate color reaction. They are the sugars that provide energy. The dissociation of carboxylic acid is represented as: 2. The stereo-selectivity of acylase I, an enzyme, for a 50:50 racemic mixture of 1-phenylethanol was determined.
Record your observations then wash test tubes thoroughly. Background Organic compounds are, by definition, any chemical compound containing carbon. Conclusion The exercises for carbohydrates mostly resulted in the former hypothesis. The results of this were also conclusive. To test for lipids, divide the piece of brown paper into 9 equal sections. The hypothesis was that the potato contained starch and the onion did not. We decided to test an egg, a common food for many and pertinent to our projects final outcome.
Polysaccharides are composed of many glucose monomers ex; starch, glycogen and cellules. The second test on proteins consisted of cleaning four test tubes and labeling them 1 through 4. Carbohydrates give energy to cells when consumed. In this lab, these solutions will be put to the test. The basis for this test is that starch is a coiled polymer of glucose. In a solution of pH 8 or higher the sugar is capable of reducing certain weak oxidizing agents such as cupric hydroxide along with a resultant oxidation of the carbonyl group of the sugar. Note: With benzaldehyde the pink colour developes slowly.
In order to confirm the results of our study we suggest that others reading this lab report conduct the experiment themselves. Organic chemistry is the science that deals with all aspects of organic compounds. As base strength increases, pKb decreases. Amino acid and albumin reacted with Ninhydrin, proof that they contained amino acid or protein. A more polar molecule will adhere more strongly to the more polar stationary phase than a less polar molecule. Rub a small amount of each substance onto its corresponding section. Place 350 µl drops of the 1% sugar solution in each test tube.
When alcohol is warmed with sodium hydroxide solution and iodine, a yellow precipitate of iodoform is formed. The result would bring out a specific color change in the macromolecule. Your challenge is to identify the unknownís chemical features and suggest a possible structure as best you can by its test results. What do the results tell you about the chemical characteristics of your unknown? Nitrous acid Test Primary aliphatic amines react with nitrous acid to produce nitrogen gas which is seen as bubbles. Methods This lab was completed at New Tech High Coppell in Mrs.